CD45RO Monoklonaler Antikörper

CD45RO Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

UCHL1

Kat-Nr. : CL488-65150

Synonyme

B220, CD45R, CD45RO, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200, UCHL1

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Galerie der Validierungsdaten

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  IF Staining of human peripheral blood mononuclear cells using CL488-65150

  Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using APC Anti-Human CD45RA (APC-65108, Clone: HI100) and CoraLite® Plus 488-conjugated Anti-Human CD45RO (CL488-65150, Clone: UCHL1) at dilution of 1:300.

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  FC experiment of human PBMCs using CL488-65150

  1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD45RA and 5 ul CoraLite® Plus 488 Anti-Human CD45RO (CL488-65150, Clone:UCHL1), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using CL488-65150

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD45RO (CL488-65150, Clone:UCHL1), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humane mononukleäre Zellen aus peripherem Blut
• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:150-1:600

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65150 bindet in IF, FC CD45RO und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-2-abhängige T-Zelllinie, CA1
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC014239
• Gene symbol: PTPRC
• Gene ID (NCBI): 5788
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).