CD45RA Monoklonaler Antikörper

CD45RA Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

APC Fluorescent Dye

CloneNo.

HI100

Kat-Nr. : APC-65108

Synonyme

B220, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200



Geprüfte Anwendungen

Erfolgreiche Detektion in IFhumane mononukleäre Zellen aus peripherem Blut
Erfolgreiche Detektion in FChumane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:150-1:600
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65108 bindet in IF, FC CD45RA und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name protein tyrosine phosphatase, receptor type, C
GenBank-ZugangsnummerBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
Konjugation APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protokolle

Produktspezifische Protokolle
IF protocol for APC CD45RA antibody APC-65108Protokoll herunterladen
FC protocol for APC CD45RA antibody APC-65108Protokoll herunterladen
Standard-Protokolle
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