CD45RA Monoklonaler Antikörper
CD45RA Monoklonal Antikörper für FC, IF
Wirt / Isotyp
Maus / IgG2b, kappa
Getestete Reaktivität
human
Anwendung
IF, FC
Konjugation
APC Fluorescent Dye
CloneNo.
HI100
Kat-Nr. : APC-65108
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in IF | humane mononukleäre Zellen aus peripherem Blut |
Erfolgreiche Detektion in FC | humane Lymphozyten aus peripherem Blut |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Immunfluoreszenz (IF) | IF : 1:150-1:600 |
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
APC-65108 bindet in IF, FC CD45RA und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Maus / IgG2b, kappa |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | k.A. |
Vollständiger Name | protein tyrosine phosphatase, receptor type, C |
GenBank-Zugangsnummer | BC014239 |
Gene symbol | PTPRC |
Gene ID (NCBI) | 5788 |
Konjugation | APC Fluorescent Dye |
Excitation/Emission maxima wavelengths | 650 nm / 660 nm |
Form | Liquid |
Reinigungsmethode | Affinitätsreinigung |
Lagerungspuffer | Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2. |
Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Hintergrundinformationen
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).
Protokolle
Produktspezifische Protokolle | |
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IF protocol for APC CD45RA antibody APC-65108 | Protokoll herunterladen |
FC protocol for APC CD45RA antibody APC-65108 | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |