CD45 Monoklonaler Antikörper

CD45 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

HI30

Kat-Nr. : APC-65109

Synonyme

B220, CD45, CD45R, GP180, HI30, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

---

Galerie der Validierungsdaten

• 

  FC experiment of human PBMCs using APC-65109

  1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD45 (APC-65109, Clone:HI30) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using APC-65109

  1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD45 (APC-65109, Clone:HI30) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of Raji using APC-65109

  1X10^6 Raji cells were surface stained with 5 ul APC Anti-Human CD45 (APC-65109, Clone: HI30) (red), or 5 ul Control Antibody. Cells were not fixed .

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs, Raji-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65109 bindet in FC CD45 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human peripheral blood leucocytes
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC014239
• Gene symbol: PTPRC
• Gene ID (NCBI): 5788
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).