CD45.1 Monoklonaler Antikörper

CD45.1 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

A20

Kat-Nr. : 65092-1-Ig

Synonyme

B220, CD45.1, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200

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Galerie der Validierungsdaten

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  FC experiment of SJL mouse splenocytes using 65092-1-Ig

  1X10^6 SJL mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45.1 (65092-1-Ig, Clone:A20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or not stained (blank) (black). Cells were not fixed .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: SJL-Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65092-1-Ig bindet in FC CD45.1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SJL-Maus-Thymozyten und -Splenozyten
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC028512
• Gene symbol: Ptprc
• Gene ID (NCBI): 19264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid, pH 7,2.
• Lagerungsbedingungen: Bei 2-8℃ lagern.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).