CD40L/CD154 Monoklonaler Antikörper

CD40L/CD154 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1E6D10

Kat-Nr. : 66502-1-Ig

Synonyme

CD154, CD40 L, CD40 ligand, CD40L, CD40L/CD154, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 66502-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66502-1-Ig (CD40L/CD154 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood platelets using 66502-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66502-1-Ig (CD40L/CD154 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, human peripheral blood platelets

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 3 publications below

Produktinformation

66502-1-Ig bindet in WB, IHC, ELISA CD40L/CD154 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD40L/CD154 fusion protein Ag24941
• Vollständiger Name: CD40 ligand
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC071754
• Gene symbol: CD40LG
• Gene ID (NCBI): 959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.

Publikationen

Species	Application	Title
human	IHC	Thyroid; A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE; Authors - YuanPing Hai
human	IHC	Pathol Res Pract; The potential role of follicular helper T cells and helper T cells type 1 in Warthin tumour.; Authors - Yoshiaki Kobayashi
human	IHC	Pathol Res Pract; The potential role of follicular helper T cells in idiopathic multicentric Castleman disease with and without TAFRO syndrome.; Authors - Nozomu Kurose