CD40 Monoklonaler Antikörper

CD40 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / Mouse IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

G28.5

Kat-Nr. : CL488-65103

Synonyme

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

---

Galerie der Validierungsdaten

• 

  FC experiment of human peripheral blood lymphocytes using CL488-65103

  1X10^6 human peripheral blood lymphocytes were surface stained with 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD40 (CL488-65103, Clone: G28.5) (red) or isotype control antibody (blue). Cells were not fixed.

• 

  FC experiment of Raji using CL488-65103

  1X10^6 Raji cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD40 (CL488-65103, Clone:G28.5) (red), or 5 ul Control Antibody. Cells were not fixed .

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Lymphozyten aus peripherem Blut, Raji-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65103 bindet in FC CD40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / Mouse IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD40 molecule, TNF receptor superfamily member 5
• Berechnetes Molekulargewicht: 277 aa, 31 kDa
• GenBank-Zugangsnummer: BC012419
• Gene symbol: CD40
• Gene ID (NCBI): 958
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen.

Hintergrundinformationen

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).