CD40 Monoklonaler Antikörper

CD40 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C10

Kat-Nr. : CL488-65062

Synonyme

AI326936, Bp50, Cd40, CD40 antigen, GP39, HIGM1, IGM, IMD3, p50, T BAM, Tnfrsf5

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Galerie der Validierungsdaten

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  FC experiment of C57BL/6 mouse splenocytes using CL488-65062

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 2 ug CoraLite® Plus 488 Anti-Mouse CD40 (CL488-65062, Clone:1C10) or 2 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: C57BL/6 mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65062 bindet in FC CD40 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: sCD40
• Vollständiger Name: CD40 antigen
• GenBank-Zugangsnummer: BC029254
• Gene symbol: Cd40
• Gene ID (NCBI): 21939
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).