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CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

SK3

Kat-Nr. : APC-65147

Synonyme

CD4, CD4 molecule, CD4mut, SK3

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using APC Anti-Human CD4 (SK3) (APC-65147)

    FC experiment of human peripheral blood lymphocytes using APC-65147

    1X10^6 human peripheral blood lymphocytes were surface stained with FITC Anti-Human CD3 (FITC-65151, clone UCHT1) and 5.00 ul/test APC Anti-Human CD4 (APC-65147, Clone:SK3). Sample were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using APC Anti-Human CD4 (SK3) (APC-65147)

    FC experiment of human peripheral blood lymphocytes using APC-65147

    1X10^6 human peripheral blood lymphocytes were surface stained with FITC Anti-Human CD3 (FITC-65151, clone UCHT1) and APC mouse IgG1 isotype control antibody. Sample were not fixed.

Geprüfte Anwendungen

Erfolgreiche Detektion in FChumane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65147 bindet in FC CD4 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name CD4 molecule
Berechnetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Konjugation APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protokolle

Produktspezifische Protokolle
FC protocol for APC CD4 antibody APC-65147Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Macarena Lucia (Verified Customer) (09-28-2022)

Good staining at recommended dilution

  • Applications: Flow Cytometry
  • Cell Tissue Type: primary T cell