CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (3)

Anwendung

IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

42

CloneNo.

2H4A2

Rezensionen (1)

Kat-Nr. : 67786-1-Ig

Synonyme

CD4, CD4 molecule, CD4mut

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Galerie der Validierungsdaten

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  IHC staining of human tonsillitis using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 67786-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD4 antibody (67786-1-Ig, Clone: 2H4A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolongewebe, humanes Lebergewebe, humanes Thymusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:450-1:1800
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 23 publications below
• IF: See 18 publications below
• FC: See 1 publications below

Produktinformation

67786-1-Ig bindet in IHC, IF, FC, ELISA CD4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD4 fusion protein Ag30870
• Vollständiger Name: CD4 molecule
• Berechnetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC025782
• Gene symbol: CD4
• Gene ID (NCBI): 920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publikationen

Species	Application	Title
human,mouse	IF	J Med Virol; Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy; Authors - Xinyue Deng
mouse	IF	Cardiovasc Res; Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism; Authors - Yang Lu
human	IHC	Clin Transl Med; Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy; Authors - Qing Wang
human,mouse	IHC	Front Immunol; LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma; Authors - Houhong Wang
human	IHC	Front Immunol; Characteristics of the immunogenicity and tumor immune microenvironment in HER2-amplified lung adenocarcinoma; Authors - Qinyang Wang
mouse	IF	Immunology; Deubiquitinase inhibitor PR-619 potentiates colon cancer immunotherapy by inducing ferroptosis; Authors - Jingjing Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Used for IF on FFPE prostate tissue. Antigen retrieval Tris-EDTA pH 9.0.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate