• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

Unkonjugiert

CloneNo.

RPA-T4

Kat-Nr. : 65143-1-Ig

Synonyme

CD4, CD4 molecule, CD4mut

Galerie der Validierungsdaten

Filter:
  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using Anti-Human CD4 (RPA-T4) (65143-1-Ig)

    FC experiment of human peripheral blood lymphocytes using 65143-1-Ig

    1X10^6 human peripheral blood lymphocytes were surface stained with 1 ug Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 1 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

Geprüfte Anwendungen

Erfolgreiche Detektion in FChumane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 1 publications below

Produktinformation

65143-1-Ig bindet in IF, FC CD4 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name CD4 molecule
Berechnetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer Phosphatbasierter Puffer mit 0,09% Natriumazid, pH 7,2.
LagerungsbedingungenBei 2-8℃ lagern.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protokolle

Produktspezifische Protokolle
FC protocol for CD4 antibody 65143-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Allergy Asthma Immunol Res

TET2 Regulates 5-Hydroxymethylcytosine Signature and CD4+ T-Cell Balance in Allergic Rhinitis.

Authors - Lu Tan
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sunil (Verified Customer) (08-03-2023)

worked as expected for conjugating our custom dyes to

  • Applications: Flow Cytometry
FH

Sareena (Verified Customer) (06-02-2023)

Worked well with compensation beads

  • Applications: Immunofluorescence, Flow Cytometry
FH

Sareena (Verified Customer) (04-20-2023)

Worked well with conjugating fluorophores and tested with compensation beads for flow cytometry analysis.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 100 ug
  • Cell Tissue Type: CD4 RPA-T4 antibody