CD38 Monoklonaler Antikörper

CD38 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HB7

Kat-Nr. : CL488-65107

Synonyme

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

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Galerie der Validierungsdaten

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  IF Staining of human peripheral blood mononuclear cells using CL488-65107

  Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD38 (CL488-65107, Clone:HB7) at dilution of 1:400.

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  FC experiment of human peripheral blood lymphocytes using CL488-65107

  100 ul human peripheral blood were surface stained 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD38 (CL488-65107, Clone:HB7) (red) or isotype control antibody (blue). Lymphocytes were gated for analysis. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humane mononukleäre Zellen aus peripherem Blut
• Erfolgreiche Detektion in FC: humane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65107 bindet in IF, FC CD38 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane BJAB-B-Zelllinie
• Vollständiger Name: CD38 molecule
• Berechnetes Molekulargewicht: 300 aa, 34 kDa
• GenBank-Zugangsnummer: BC007964
• Gene symbol: CD38
• Gene ID (NCBI): 952
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).