CD38 Polyklonaler Antikörper

CD38 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 25284-1-AP

Synonyme

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

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Galerie der Validierungsdaten

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  WB analysis using 25284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25284-1-AP (CD38 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 25284-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25284-1-AP (CD38 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 25284-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 25284-1-AP (CD38 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HL-60 using 25284-1-AP

  1X10^6 HL-60 cells were stained with 0.2ug CD38 antibody (25284-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, HL-60-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Appendizitis-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HL-60-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

25284-1-AP bindet in WB, IHC, IF, FC, ELISA CD38 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD38 fusion protein Ag17868
• Vollständiger Name: CD38 molecule
• Berechnetes Molekulargewicht: 300 aa, 34 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC007964
• Gene symbol: CD38
• Gene ID (NCBI): 952
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Publikationen

Species	Application	Title
human	IHC	Front Oncol; CD38 Multi-Functionality in Oral Squamous Cell Carcinoma: Prognostic Implications, Immune Balance, and Immune Checkpoint.; Authors - Zhuang Ding
human	IF	Cell Commun Signal; TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma.; Authors - Hua Bai
human	WB,IHC	Oncol Rep; CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer.; Authors - Shan Liao
human	WB	PLoS Pathog; The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness; Authors - Elena Sugrue