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CD37 Polyklonaler Antikörper

CD37 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21044-1-AP

Synonyme

CD37, CD37 molecule, GP52 40, Leukocyte antigen CD37, Tetraspanin 26, Tspan 26, TSPAN26

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of rat spleen tissue using CD37 Polyclonal antibody (21044-1-AP)

    WB analysis of rat spleen using 21044-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 21044-1-AP (CD37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using CD37 Polyclonal antibody (21044-1-AP)

    WB analysis of human spleen using 21044-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 21044-1-AP (CD37 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CD37 Polyclonal antibody (21044-1-AP)

    WB analysis of HeLa using 21044-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21044-1-AP (CD37 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD37 Polyclonal antibody (21044-1-AP)

    IHC staining of human tonsillitis using 21044-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21044-1-AP (CD37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD37 Polyclonal antibody (21044-1-AP)

    IHC staining of human tonsillitis using 21044-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21044-1-AP (CD37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD37 Polyclonal antibody (21044-1-AP)

    IHC staining of human spleen using 21044-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen slide using 21044-1-AP (CD37 Antibody) at dilution of 1:50.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBRattenmilzgewebe, HeLa-Zellen, humanes Milzgewebe
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe, humanes Milzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IHCSee 2 publications below
IFSee 1 publications below

Produktinformation

21044-1-AP bindet in WB, IHC, IF, ELISA CD37 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD37 fusion protein Ag15384
Vollständiger Name CD37 molecule
Berechnetes Molekulargewicht 281 aa, 32 kDa
Beobachtetes Molekulargewicht 40-52 kDa
GenBank-ZugangsnummerBC110379
Gene symbol CD37
Gene ID (NCBI) 951
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).

Protokolle

Produktspezifische Protokolle
WB protocol for CD37 antibody 21044-1-APProtokoll herunterladen
IHC protocol for CD37 antibody 21044-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC,IF

iScience

The clinical features, prognostic significance, and immune heterogeneity of CD37 in diffuse gliomas.

Authors - Xuejun Yan
View Article
mouseIHC

Sci Rep

89Zr-PET imaging to predict tumor uptake of 177Lu-NNV003 anti-CD37 radioimmunotherapy in mouse models of B cell lymphoma.

Authors - Danique Giesen
View Article