CD357 (GITR) Monoklonaler Antikörper

CD357 (GITR) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

DTA-1

Kat-Nr. : CL488-65102

Synonyme

AITR, CD357, CD357 (GITR), Gitr, Tnfrsf18

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using CL488-65102

  1X10^6 mouse splenocytes were surface stained with PE-Anti-Mouse CD4 (PE-65104, Clone: GK1.5), CoraLite® Plus 647-Conjugated Anti-Mouse CD25 (CL647-65137, Clone: PC61.5) and 5 ul CoraLite® Plus 488 Anti-Mouse CD357 (GITR) (CL488-65102, Clone: DTA-1). Cells were not fixed. CD4+ lymphocytes were gated for analysis of CD25 and CD357 (GITR) staining.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65102 bindet in FC CD357 (GITR) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-CD25+-CD4+-T-Zellen
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 18
• GenBank-Zugangsnummer: BC146517
• Gene symbol: Tnfrsf18
• Gene ID (NCBI): 21936
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).