CD35 Monoklonaler Antikörper

CD35 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

E11

Kat-Nr. : CL555-65254

Synonyme

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using CL555-65254

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD35 (CL555-65254, Clone:E11) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL555-65254

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD35 (CL555-65254, Clone:E11) (red) or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-65254 bindet in FC CD35 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human CD35 from Acute monocytic leukemia cells and normal blood monocytes
• Vollständiger Name: complement component (3b/4b) receptor 1 (Knops blood group)
• Berechnetes Molekulargewicht: 224 kDa
• GenBank-Zugangsnummer: NM_000573
• Gene symbol: CR1
• Gene ID (NCBI): 1378
• Konjugation: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.