CD35 Monoklonaler Antikörper

CD35 Monoklonal Antikörper für FC, ELISA

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

E11

Kat-Nr. : 65254-1-Ig

Synonyme

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using 65254-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD35 (65254-1-Ig, Clone:E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65254-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD35 (65254-1-Ig, Clone:E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65254-1-Ig bindet in FC, ELISA CD35 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human CD35 from Acute monocytic leukemia cells and normal blood monocytes
• Vollständiger Name: complement component (3b/4b) receptor 1 (Knops blood group)
• Berechnetes Molekulargewicht: 224 kDa
• GenBank-Zugangsnummer: NM_000573
• Gene symbol: CR1
• Gene ID (NCBI): 1378
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.