CD33 Monoklonaler Antikörper

CD33 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3G4

Kat-Nr. : 67135-2-Ig

Synonyme

CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3

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Galerie der Validierungsdaten

• 

  WB analysis of Mo7e using 67135-2-Ig

  Mo7e cells were subjected to SDS PAGE followed by western blot with 67135-2-Ig (CD33 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 67135-2-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 67135-2-Ig (CD33 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67135-2-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67135-2-Ig (CD33 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mo7e cells, THP-1-Zellen, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67135-2-Ig bindet in WB, ELISA CD33 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD33 fusion protein Ag11402
• Vollständiger Name: CD33 molecule
• Berechnetes Molekulargewicht: 364 aa, 40 kDa
• Beobachtetes Molekulargewicht: 67-70 kDa
• GenBank-Zugangsnummer: BC028152
• Gene symbol: CD33
• Gene ID (NCBI): 945
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.