CD31 Monoklonaler Antikörper

CD31 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3F8E2

Kat-Nr. : CL594-66065

Synonyme

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

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Galerie der Validierungsdaten

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  IF Staining of human tonsillitis using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 2A1E2 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 2A1E2 ) at dilution of 1:200.

• 

  IF Staining of human placenta using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 3F8E2 ) at dilution of 1:200.

• 

  IF Staining of human placenta using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 3F8E2 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe, humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66065 bindet in IF CD31 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD31 fusion protein Ag1787
• Vollständiger Name: platelet/endothelial cell adhesion molecule
• Berechnetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC022512
• Gene symbol: PECAM1
• Gene ID (NCBI): 5175
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.