CD31 Monoklonaler Antikörper
CD31 Monoklonal Antikörper für FC, IF
Wirt / Isotyp
Ratte / IgG2a, kappa
Getestete Reaktivität
Maus
Anwendung
IF, FC
Konjugation
CoraLite®594 Fluorescent Dye
CloneNo.
390
Kat-Nr. : CL594-65058
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in IF | bEnd.3-Zellen |
Erfolgreiche Detektion in FC | Maus-Splenozyten |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Immunfluoreszenz (IF) | IF : 1:50-1:500 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL594-65058 bindet in IF, FC CD31 und zeigt Reaktivität mit Maus
Getestete Reaktivität | Maus |
Wirt / Isotyp | Ratte / IgG2a, kappa |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | C3H/HeJ Maus hämatopoetische Vorläuferzelllinie 32D |
Vollständiger Name | platelet/endothelial cell adhesion molecule 1 |
GenBank-Zugangsnummer | BC008519 |
Gene symbol | Pecam1 |
Gene ID (NCBI) | 18613 |
Konjugation | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Reinigungsmethode | Affinitätsreinigung |
Lagerungspuffer | PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3. |
Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Hintergrundinformationen
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
Protokolle
Produktspezifische Protokolle | |
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IF protocol for CL594 CD31 antibody CL594-65058 | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |