CD31 Monoklonaler Antikörper

CD31 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

390

Kat-Nr. : CL594-65058

Synonyme

C85791, Cd31, Pecam, PECAM 1, Pecam1



Geprüfte Anwendungen

Erfolgreiche Detektion in IFbEnd.3-Zellen
Erfolgreiche Detektion in FCMaus-Splenozyten

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-65058 bindet in IF, FC CD31 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Ratte / IgG2a, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen C3H/HeJ Maus hämatopoetische Vorläuferzelllinie 32D
Vollständiger Name platelet/endothelial cell adhesion molecule 1
GenBank-ZugangsnummerBC008519
Gene symbol Pecam1
Gene ID (NCBI) 18613
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 CD31 antibody CL594-65058Protokoll herunterladen
Standard-Protokolle
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