CD31 Monoklonaler Antikörper

CD31 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

390

Rezensionen (1)

Kat-Nr. : CL488-65058

Synonyme

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Galerie der Validierungsdaten

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  IF Staining of bEnd.3 using CL488-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite® Plus 488 CD31 antibody (CL488-65058, Clone: 390 ) at dilution of 1:100.

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  FC experiment of BALB/C mouse splenocytes using CL488-65058

  1X10^6 BALB/C mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD31 (CL488-65058, Clone:390), or 0.5 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: bEnd.3-Zellen
• Erfolgreiche Detektion in FC: BALB/c Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65058 bindet in IF, FC CD31 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C3H/HeJ Maus hämatopoetische Vorläuferzelllinie 32D
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• GenBank-Zugangsnummer: BC008519
• Gene symbol: Pecam1
• Gene ID (NCBI): 18613
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vanessa (Verified Customer) (09-27-2023)

Label but not very bright. Hard to distinguish from background.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Brain