CD31 Monoklonaler Antikörper

CD31 Monoklonal Antikörper für IF,FC

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

390

Kat-Nr. : 65058-1-Ig

Synonyme

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Galerie der Validierungsdaten

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  IF Staining of bEnd.3 using 65058-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (65058-1-Ig, Clone: 390 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rat IgG(H+L).

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  FC experiment of mouse splenocytes using 65058-1-Ig

  1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD31 (65058-1-Ig, Clone:390) and FITC anti-rat IgG2a Antibody at dilution 1:50 (green), or stained with 0.5 ug rat IgG2a isotype control and FITC anti-rat IgG2a Antibody at dilution 1:50 (black). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: bEnd.3-Zellen
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below

Produktinformation

65058-1-Ig bindet in IF, FC CD31 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C3H/HeJ Maus hämatopoetische Vorläuferzelllinie 32D
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• GenBank-Zugangsnummer: BC008519
• Gene symbol: Pecam1
• Gene ID (NCBI): 18613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Borat-gepufferte Kochsalzlösung, pH 8,2.
• Lagerungsbedingungen: Bei 2-8℃ lagern.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publikationen

Species	Application	Title
	IF	mBio; Human ApoE2 protects mice against Plasmodium berghei ANKA experimental cerebral malaria; Authors - Rui Liang
mouse	IF	J Ethnopharmacol; Berberis dictyophylla F. inhibits angiogenesis and apoptosis of diabetic retinopathy via suppressing HIF-1α/VEGF/DLL-4/Notch-1 pathway.; Authors - Xiaopeng Ai
mouse	IF	Adv Mater; Photothermal and Photocatalytic Glycol Chitosan and Polydopamine-Grafted Oxygen Vacancy Bismuth Oxyiodide (BiO1-x I) Nanoparticles for the Diagnosis and Targeted Therapy of Diabetic Wounds; Authors - Jianglong Kong