CD300A Monoklonaler Antikörper

CD300A Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F9C5

Kat-Nr. : 67242-1-Ig

Synonyme

CD300A, CD300a molecule, CLM 8, CMRF 35 H9, CMRF 35H, CMRF35 H, CMRF35 H9, CMRF35 like molecule 8, CMRF35H, CMRF35H9, IGSF12, Inhibitory receptor protein 60, IRC1, IRC1/IRC2, IRC2, IRp60, NK inhibitory receptor

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Galerie der Validierungsdaten

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  WB analysis of THP-1 using 67242-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67242-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 67242-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 67242-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67242-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 67242-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of THP-1 using 67242-1-Ig

  1X10^6 THP-1 cells were surface stained with 0.4 ug Anti-Human CD300A (67242-1-Ig, Clone:2F9C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HL-60-Zellen, humanes Milzgewebe, Jurkat-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche Detektion in FC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67242-1-Ig bindet in WB, FC, ELISA CD300A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD300A fusion protein Ag5776
• Vollständiger Name: CD300a molecule
• Berechnetes Molekulargewicht: 299 aa, 33 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC032352
• Gene symbol: CD300A
• Gene ID (NCBI): 11314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.