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CD3 gamma Monoklonaler Antikörper

CD3 gamma Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2E9G7

Kat-Nr. : CL594-60347

Synonyme

CD3 GAMMA, CD3G, T cell receptor T3 gamma chain, T3G

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD3 gamma Monoclonal antib (CL594-60347)

    IF Staining of human tonsillitis using CL594-60347

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD3 gamma antibody (CL594-60347, Clone: 2E9G7 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD3 gamma Monoclonal antib (CL594-60347)

    IF Staining of human tonsillitis using CL594-60347

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD3 gamma antibody (CL594-60347, Clone: 2E9G7 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFhumanes Tonsillitisgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60347 bindet in IF CD3 gamma und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen CD3 gamma fusion protein Ag15502
Vollständiger Name CD3g molecule, gamma (CD3-TCR complex)
Berechnetes Molekulargewicht 182 aa, 20 kDa
Beobachtetes Molekulargewicht 20-25 kDa
GenBank-ZugangsnummerBC113830
Gene symbol CD3G
Gene ID (NCBI) 917
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 CD3 gamma antibody CL594-60347Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen