CD3 Monoklonaler Antikörper

CD3 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

17A2

Kat-Nr. : CL488-65077

Synonyme

AI504783, CD3, Cd3e, CD3epsilon, T3e

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Galerie der Validierungsdaten

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  IF Staining of mouse splenocytes using CL488-65077

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD3 (CL488-65077, Clone: 17A2) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL488-65077

  1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5) and CoraLite® Plus 488-Rat IgG2b isotype control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) (right). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:250-1:1000

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65077 bindet in IF, FC CD3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: γδTCR-positive T-T hybridoma D1
• Vollständiger Name: CD3 antigen, epsilon polypeptide
• GenBank-Zugangsnummer: BC098236
• Gene symbol: Cd3e
• Gene ID (NCBI): 12501
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).