CD3 Monoklonaler Antikörper
CD3 Monoklonal Antikörper für FC
Wirt / Isotyp
Maus / IgG1, kappa
Getestete Reaktivität
human
Anwendung
FC
Konjugation
CoraLite® Plus 405 Fluorescent Dye
CloneNo.
UCHT1
Kat-Nr. : CL405-65151
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in FC | humane PBMCs |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL405-65151 bindet in FC CD3 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Maus / IgG1, kappa |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | k.A. |
Vollständiger Name | CD3e molecule, epsilon (CD3-TCR complex) |
Berechnetes Molekulargewicht | 207 aa, 23 kDa |
GenBank-Zugangsnummer | BC049847 |
Gene symbol | CD3E |
Gene ID (NCBI) | 916 |
Konjugation | CoraLite® Plus 405 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 399 nm / 422 nm |
Form | Liquid |
Reinigungsmethode | Affinitätsreinigung |
Lagerungspuffer | PBS with 0.1% sodium azide and 0.5% BSA. |
Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Hintergrundinformationen
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protokolle
Produktspezifische Protokolle | |
---|---|
FC protocol for CL Plus 405 CD3 antibody CL405-65151 | Protokoll herunterladen |
Standard-Protokolle | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |