CD3 Rekombinanter Antikörper

CD3 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

OKT3

Kat-Nr. : 65504-1-RR

Synonyme

CD3E, T3E, TCRE

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using 65504-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 Rabbit Recombinant Antibody (65504-1-RR, Clone:OKT3) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD45. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65504-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 Rabbit Recombinant Antibody (65504-1-RR, Clone:OKT3) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65504-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 Rabbit Recombinant Antibody (65504-1-RR, Clone:OKT3) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.2ug Anti-Human CD3 Mouse Monoclonal Antibdody (Clone:OKT3) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD45. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65504-1-RR bindet in FC CD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human T cells
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3E
• Gene ID (NCBI): 916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).