CD3ε Monoklonaler Antikörper

CD3ε Monoklonal Antikörper für FC, IF

Wirt / Isotyp

hamster / IgG

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

Unkonjugiert

CloneNo.

145-2C11

Rezensionen (1)

Kat-Nr. : 65060-1-Ig

Synonyme

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e

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Galerie der Validierungsdaten

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  IF Staining of mouse splenocytes using 65060-1-Ig

  Immunofluorescent analysis of mouse splenocytes using CD3ε antibody (65060-1-Ig, Clone: 145-2C11 ) at dilution of 1:500 and FITC Goat anti-Hamster(Armenian) IgG.

• 

  FC experiment of C57BL/6 mouse splenocytes using 65060-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and 0.5 ug Anti-Mouse CD3ε (65060-1-Ig, Clone:145-2C11) or 0.5 ug Armenian Hamster IgG Isotype Control, and FITC anti-Armenian Hamster IgG antibody at dilution 1:100. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Splenozyten
• Erfolgreiche Detektion in FC: C57BL/6 mouse splenocytes

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65060-1-Ig bindet in IF, FC CD3ε und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: H-2Kb-spezifischer muriner cytotoxischer T-Lymphozyten (CTL) -Klon BM10-37
• Vollständiger Name: CD3 antigen, epsilon polypeptide
• GenBank-Zugangsnummer: BC098236
• Gene symbol: Cd3e
• Gene ID (NCBI): 12501
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Helena (Verified Customer) (07-22-2022)

Not many t cells to detect but they were observable in this tissue section.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain tissue post stroke

CD3ε Antibody Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in mouse brain tissue post stroke (Cat no:65060-1-Ig)