PD-1/CD279 Monoklonaler Antikörper

PD-1/CD279 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

Publikationen

1

CloneNo.

RMP1-30

Kat-Nr. : APC-65142

Synonyme

CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1

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Galerie der Validierungsdaten

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  FC experiment of BALB/C mouse splenocytes using APC-65142

  1X10^6 unstimulated or anti-CD3/CD28 treated (2 days) BALB/C mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse PD-1/CD279 (APC-65142, Clone: RMP1-30), and 0.5 ug FITC Plus Anti-Mouse CD3 (17A2) (FITC-65077, Clone: 17A2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated 2 days BALB/C mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

APC-65142 bindet in FC PD-1/CD279 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cells
• Vollständiger Name: programmed cell death 1
• GenBank-Zugangsnummer: BC119179
• Gene symbol: Pdcd1
• Gene ID (NCBI): 18566
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

Publikationen

Species	Application	Title
mouse	FC	Stem Cell Res Ther; Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment.; Authors - Qing Zhang