CD274 (PD-L1, B7-H1) Monoklonaler Antikörper

CD274 (PD-L1, B7-H1) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

Publikationen

1

CloneNo.

10F.9G2

Kat-Nr. : APC-65073

Synonyme

B7 H1, Cd274, CD274 (PD-L1, B7-H1), CD274 antigen, PD L1, Pdcd1l1, Pdcd1lg1

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Galerie der Validierungsdaten

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  FC experiment of BALB/C mouse splenocytes using APC-65073

  1X10^6 unstimulated or anti-CD3/CD28 treated (2 days) BALB/C mouse splenocytes were surface stained with FITC Plus Anti-Mouse CD3 (17A2) and 0.2 ug APC Anti-Mouse CD274 (PD-L1, B7-H1) (APC-65073, Clone: 10F.9G2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated 2 days BALB/C mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

APC-65073 bindet in FC CD274 (PD-L1, B7-H1) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD274 antigen
• GenBank-Zugangsnummer: BC066841
• Gene symbol: Cd274
• Gene ID (NCBI): 60533
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Publikationen

Species	Application	Title
	FC	Cell Signal; Cancer-associated fibroblasts-induced remodeling of tumor immune microenvironment via Jagged1 in glioma; Authors - Qing Zhang