CD25 Monoklonaler Antikörper

CD25 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG1, lambda

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

Publikationen

3

CloneNo.

PC61.5

Kat-Nr. : APC-65137

Synonyme

CD25, Il2r, Il2ra, Ly 43

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Galerie der Validierungsdaten

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  FC experiment of BALB/C mouse splenocytes using APC-65137

  1X10^6 untreated or anti-CD3/CD28 treated (2 days) mouse splenocytes were co-surface stained with FITC Plus Anti-Mouse CD4 (GK1.5) and 0.25 ug APC Anti-Mouse CD25 (APC-65137, Clone:PC61.5) or 0.25 ug APC Rat IgG1 Isotype Control (HRPN). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated 2days mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 3 publications below

Produktinformation

APC-65137 bindet in FC CD25 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: interleukin 2 receptor, alpha chain
• GenBank-Zugangsnummer: BC114437
• Gene symbol: Il2ra
• Gene ID (NCBI): 16184
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

Publikationen

Species	Application	Title
mouse	FC	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
mouse	FC	Acta Biomater; Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment; Authors - Zhuo Cheng
mouse	FC	J Neuroinflammation; 27-hydroxycholesterol causes cognitive deficits by disturbing Th17/Treg balance and the related immune responses in mild cognitive impairment patients and C57BL/6J mice; Authors - Tao Wang