CD25 Monoklonaler Antikörper

CD25 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG1, lambda

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Atlantic Blue™ Fluorescent Dye

CloneNo.

PC61.5

Kat-Nr. : AB-65137

Synonyme

CD25, Il2r, Il2ra, Ly 43

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using AB-65137

  1X10^6 plate-bound Anti-CD3 and Anti-CD28 stimulated mouse splenocytes were surface stained with 0.5 ug Atlantic Blue™ Anti-Mouse CD25 (AB-65137, Clone: PC61.5) (red), or 0.5 ug Control Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: plate-bound Anti-CD3 and Anti-CD28 stimulated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

AB-65137 bindet in FC CD25 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: interleukin 2 receptor, alpha chain
• GenBank-Zugangsnummer: BC114437
• Gene symbol: Il2ra
• Gene ID (NCBI): 16184
• Konjugation: Atlantic Blue™ Fluorescent Dye
• Excitation/Emission maxima wavelengths: 404 nm / 458 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).