CD25 Monoklonaler Antikörper

CD25 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

BC96

Kat-Nr. : 65096-1-Ig

Synonyme

CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR

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Galerie der Validierungsdaten

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  FC experiment of human peripheral blood lymphocytes using 65096-1-Ig

  1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD4 (APC-65143, Clone: RPA-T4), 1 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using 65096-1-Ig

  1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD4 (APC-65143, Clone: RPA-T4), 1 ug Mouse IgG1 Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

65096-1-Ig bindet in FC CD25 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: interleukin 2 receptor, alpha
• Berechnetes Molekulargewicht: 272 aa, 31 kDa
• GenBank-Zugangsnummer: BC156627
• Gene symbol: IL2RA
• Gene ID (NCBI): 3559
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid, pH 7,2.
• Lagerungsbedingungen: Bei 2-8℃ lagern.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

Publikationen

Species	Application	Title
human	FC	DNA Cell Biol; Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis.; Authors - Yuan-Hao Wu