CD24 Monoklonaler Antikörper

CD24 Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1H5C4

Kat-Nr. : 67627-1-Ig

Synonyme

CD24, CD24 molecule, CD24A, FLJ22950, FLJ43543, Signal transducer CD24

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Galerie der Validierungsdaten

• 

  WB analysis using 67627-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Ramos using 67627-1-Ig

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human CD24 (67627-1-Ig, Clone:1H5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Daudi-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in FC: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67627-1-Ig bindet in WB, FC, ELISA CD24 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD24 fusion protein Ag11679
• Vollständiger Name: CD24 molecule
• Berechnetes Molekulargewicht: 8 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC007674
• Gene symbol: CD24
• Gene ID (NCBI): 100133941
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

Publikationen

Species	Application	Title
human	WB	Breast Cancer (Dove Med Press); CD24 May Serve as an Immunotherapy Target in Triple-Negative Breast Cancer by Regulating the Expression of PD-L1; Authors - Xudong Zhu