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CD23 Monoklonaler Antikörper

CD23 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

4C7C9

Kat-Nr. : CL594-60208

Synonyme

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human tonsillitis using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-60208 (CD23 antibody) at dilution of 1:50 .

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human tonsillitis using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-60208 (CD23 antibody) at dilution of 1:50 .

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human lymphoma using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CoraLite®594 CD23 antibody (CL594-60208, Clone: 4C7C9 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human lymphoma using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CoraLite®594 CD23 antibody (CL594-60208, Clone: 4C7C9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U-937 cells using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    FC experiment of U-937 using CL594-60208

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD23 (CL594-60208, Clone:4C7C9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFhumanes Tonsillitisgewebe, humanes Lymphomgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60208 bindet in IF CD23 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen CD23 fusion protein Ag0425
Vollständiger Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Berechnetes Molekulargewicht 321 aa, 36 kDa
GenBank-ZugangsnummerBC064417
Gene symbol FCER2
Gene ID (NCBI) 2208
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 CD23 antibody CL594-60208Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen