CD23 Monoklonaler Antikörper

CD23 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

3H6B8

Kat-Nr. : 60208-1-Ig

Synonyme

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

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Galerie der Validierungsdaten

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  WB analysis of DC2.4 using 60208-1-Ig

  DC2.4, Raji, Ramos and Daudi cells were subjected to SDS PAGE followed by western blot with 60208-1-Ig (CD23 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-1-Ig (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-1-Ig (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 60208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-1-Ig(CD23 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 60208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-1-Ig(CD23 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human tonsillitis using 60208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-1-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-1-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DC2.4-Zellen, Daudi-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

60208-1-Ig bindet in WB, IHC, IF, ELISA CD23 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD23 fusion protein Ag0425
• Vollständiger Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Berechnetes Molekulargewicht: 321 aa, 36 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC064417
• Gene symbol: FCER2
• Gene ID (NCBI): 2208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

Publikationen

Species	Application	Title
human	IHC,IF	Clin Transl Med; Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy; Authors - Qing Wang
human	IF	Sci Signal; Short stature and combined immunodeficiency associated with mutations in RGS10.; Authors - Ivan K Chinn
mouse	WB,IF	Biol Pharm Bull; Indomethacin, a Non-steroidal Anti-inflammatory Drug, Induces Skin Dryness via PPARγ in Mice.; Authors - Kiyoko Maruyama
human	IHC	BMC Musculoskelet Disord; Anti-signal recognition particle positive necrotizing myopathy-sjogren's syndrome overlap syndrome: a descriptive study on clinical and myopathology features; Authors - Li Xu