CD206 Monoklonaler Antikörper

CD206 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

APC Fluorescent Dye

Publikationen

1

CloneNo.

15-2

Kat-Nr. : APC-65155

Synonyme

CD206, CLEC13D, Macrophage mannose receptor 1, mannose receptor, C type 1, MMR, MRC1

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Galerie der Validierungsdaten

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  FC experiment of human monocyte-derived dendritic cells using APC-65155

  Human monocyte-derived dendritic cells (monocytes stimulated with GM-CSF and IL-4) were surface stained with 10 ul APC Anti-Human CD206 (APC-65155, Clone:15-2) (blue) or APC-Mouse IgG1 isotype control (black). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane, aus Monozyten gewonnene dendritische Zellen Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

APC-65155 bindet in FC CD206 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigter humaner Mannose-Rezeptor
• Vollständiger Name: mannose receptor, C type 1
• Berechnetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: NM_002438
• Gene symbol: MRC1
• Gene ID (NCBI): 4360
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit Allophycocyanin (APC) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

Publikationen

Species	Application	Title
	FC	Int J Mol Sci; C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis; Authors - Xinyi Qu