CD163 Rekombinanter Antikörper

CD163 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

GHI/61

Kat-Nr. : 65501-1-RR

Synonyme

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using 65501-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65501-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65501-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.2 ug Anti-Human CD163 Mouse Monoclonal Antibody (Clone:GHI/61) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65501-1-RR bindet in FC CD163 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Glykoprotein-Zubereitung aus Haarzell-Leukämie-Milz
• Vollständiger Name: CD163 molecule
• Berechnetes Molekulargewicht: 1156 aa, 125 kDa
• GenBank-Zugangsnummer: BC051281
• Gene symbol: CD163
• Gene ID (NCBI): 9332
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).