CD16/32 Monoklonaler Antikörper

CD16/32 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, lambda

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

93

Kat-Nr. : CL555-65057

Synonyme

AI528646, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using CL555-65057

  1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 1 ug CoraLite® Plus 555 Anti-Mouse CD16/32 (CL555-65057, Clone:93) or 1 ug CoraLite® Plus 555 Rat IgG2a Isotype Control (2A3) (CL555-65209, Clone: 2A3). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-65057 bindet in FC CD16/32 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VH81X Tg-B-Zellen und T220-Stromazelllinie
• Vollständiger Name: Fc receptor, IgG, low affinity IIb
• GenBank-Zugangsnummer: BC038070
• Gene symbol: Fcgr2b
• Gene ID (NCBI): 14130
• Konjugation: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.