CD16/32 Monoklonaler Antikörper

CD16/32 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, lambda

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

93

Kat-Nr. : CL405-65057

Synonyme

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1



Geprüfte Anwendungen

Erfolgreiche Detektion in FCMaus-Splenozyten

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL405-65057 bindet in FC CD16/32 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Ratte / IgG2a, lambda
Klonalität Monoklonal
Typ Antikörper
Immunogen VH81X Tg-B-Zellen und T220-Stromazelllinie
Vollständiger Name Fc receptor, IgG, low affinity IIb
GenBank-ZugangsnummerBC038070
Gene symbol Fcgr2b
Gene ID (NCBI) 14130
Konjugation CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission maxima wavelengths399 nm / 422 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 405 CD16/32 antibody CL405-65057Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen