CD16/32 Monoklonaler Antikörper

CD16/32 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, lambda

Getestete Reaktivität

Maus und mehr (1)

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

93

Kat-Nr. : 65057-1-Ig

Synonyme

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

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Galerie der Validierungsdaten

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  FC experiment of BALB/c mouse splenocytes using 65057-1-Ig

  1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD16/32 (65057-1-Ig, Clone:93) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Control Antibody. Cells were not fixed .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: BALB/c Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below
• FC: See 3 publications below

Produktinformation

65057-1-Ig bindet in IF, FC CD16/32 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Affe, Maus
• Wirt / Isotyp: Ratte / IgG2a, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VH81X Tg-B-Zellen und T220-Stromazelllinie
• Vollständiger Name: Fc receptor, IgG, low affinity IIb
• GenBank-Zugangsnummer: BC038070
• Gene symbol: Fcgr2b
• Gene ID (NCBI): 14130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.

Publikationen

Species	Application	Title
monkey	FC	Front Microbiol; Response to HIV-1 gp160-carrying recombinant virus HSV-1 and HIV-1 VLP combined vaccine in BALB/c mice; Authors - Beibei Zhang
mouse	IF	Cell Signal; OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization; Authors - Yunyun Li
mouse	IF,FC	J Nanobiotechnology; Bioengineered stem cell membrane functionalized nanoparticles combine anti-inflammatory and antimicrobial properties for sepsis treatment; Authors - Lu Lu
mouse	FC	Stem Cells Dev; Deer antler reserve mesenchyme cell-conditioned medium reduces the destruction of periodontitis in mice; Authors - Hongbing Lin
mouse	IF	BMC Neurosci; KDM4A, involved in the inflammatory and oxidative stress caused by traumatic brain injury-hemorrhagic shock, partly through the regulation of the microglia M1 polarization; Authors - Jimin Cai