CD154 Monoklonaler Antikörper

CD154 Monoklonal Antikörper für FC

Wirt / Isotyp

Armenian Hamster / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

MR1

Kat-Nr. : CL488-65129

Synonyme

CD154, CD40 ligand, Cd40l, Cd40lg, gp39, HIGM1, IGM, IMD3, Ly 62, Ly62, MR1, T BAM, Tnfsf5, TRAP

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Galerie der Validierungsdaten

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  FC experiment of BALB/c mouse CD4+ T cells using CL488-65129

  1X10^6 unstimulated or Cell Stimulation Cocktail stimulated (overnight) BALB/c mouse CD4+ T cells were surface co-stained with PE Anti-Mouse CD4 (GK1.5) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD154 (CL488-65129, Clone: MR1). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Cell Stimulation Cocktail stimulated mouse CD4+ T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65129 bindet in FC CD154 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Armenian Hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD40 ligand
• GenBank-Zugangsnummer: BC119225
• Gene symbol: Cd40lg
• Gene ID (NCBI): 21947
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.