CD146 Monoklonaler Antikörper
CD146 Monoklonal Antikörper für FC
Wirt / Isotyp
Maus / IgG1, kappa
Getestete Reaktivität
human, Hund, Kaninchen, Maus
Anwendung
FC
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
P1H12
Kat-Nr. : CL488-65181
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in FC | A375-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL488-65181 bindet in FC CD146 und zeigt Reaktivität mit human, Hund, Kaninchen, Maus
Getestete Reaktivität | human, Hund, Kaninchen, Maus |
Wirt / Isotyp | Maus / IgG1, kappa |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Gezüchtete humane Nabelschnurzellen |
Vollständiger Name | melanoma cell adhesion molecule |
Berechnetes Molekulargewicht | 646 aa, 72 kDa |
GenBank-Zugangsnummer | BC056418 |
Gene symbol | MCAM |
Gene ID (NCBI) | 4162 |
Konjugation | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Reinigungsmethode | Affinitätsreinigung |
Lagerungspuffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Hintergrundinformationen
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).
Protokolle
Produktspezifische Protokolle | |
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FC protocol for CL Plus 488 CD146 antibody CL488-65181 | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |