CD146 Monoklonaler Antikörper

CD146 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, Hund, Kaninchen, Maus

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

P1H12

Kat-Nr. : CL488-65181

Synonyme

CD146, MCAM, MUC18



Geprüfte Anwendungen

Erfolgreiche Detektion in FCA375-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65181 bindet in FC CD146 und zeigt Reaktivität mit human, Hund, Kaninchen, Maus

Getestete Reaktivität human, Hund, Kaninchen, Maus
Wirt / Isotyp Maus / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Gezüchtete humane Nabelschnurzellen
Vollständiger Name melanoma cell adhesion molecule
Berechnetes Molekulargewicht 646 aa, 72 kDa
GenBank-ZugangsnummerBC056418
Gene symbol MCAM
Gene ID (NCBI) 4162
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 CD146 antibody CL488-65181Protokoll herunterladen
Standard-Protokolle
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