CD14 Monoklonaler Antikörper

CD14 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

UCHM-1

Kat-Nr. : CL488-65056

Synonyme

CD14, CD14 molecule

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using CL488-65056

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD14 (CL488-65056, Clone:UCHM-1) (red) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL488-65056

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD14 (CL488-65056, Clone:UCHM-1) or unstained. Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65056 bindet in FC CD14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Thymozyten und Lymphozyten aus peripherem Blut eines in Remission befindlichen AML-Patienten
• Vollständiger Name: CD14 molecule
• Berechnetes Molekulargewicht: 375 aa, 40 kDa
• GenBank-Zugangsnummer: BC010507
• Gene symbol: CD14
• Gene ID (NCBI): 929
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.