CD13 Monoklonaler Antikörper

CD13 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2D8D11

Kat-Nr. : CL594-66211

Synonyme

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

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Galerie der Validierungsdaten

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  IF Staining of mouse kidney using CL594-66211

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CL594-66211 (CD13 antibody) at dilution of 1:100.

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  IF Staining of mouse kidney using CL594-66211

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CL594-66211 (CD13 antibody) at dilution of 1:100.

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  FC experiment of U-937 using CL594-66211

  1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD13 (CL594-66211, Clone:2D8D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Mausnierengewebe
• Erfolgreiche Detektion in FC: U-937-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66211 bindet in IF, FC (Intra) CD13 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD13 fusion protein Ag5976
• Vollständiger Name: alanyl (membrane) aminopeptidase
• Berechnetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC058928
• Gene symbol: ANPEP
• Gene ID (NCBI): 290
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174).And this antibody is conjugated with CL594(Ex/Em 593 nm/614 nm).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C