CD117 Monoklonaler Antikörper

CD117 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, Rind

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

104D2

Kat-Nr. : APC-65154

Synonyme

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit

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Galerie der Validierungsdaten

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  FC experiment of TF-1 using APC-65154

  1X10^6 TF-1 cells were surface stained with 10.00 ul/test APC Anti-Human CD117 (APC-65154, Clone:104D2) (red) or isotype control antibody (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: TF-1-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65154 bindet in FC CD117 und zeigt Reaktivität mit human, Rind

• Getestete Reaktivität: human, Rind
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MOLM-1 megakaryozytische Zellen
• Vollständiger Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 976 aa, 110 kDa
• GenBank-Zugangsnummer: BC071593
• Gene symbol: KIT
• Gene ID (NCBI): 3815
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit Allophycocyanin (APC) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).