CD117 (c-Kit) Monoklonaler Antikörper

CD117 (c-Kit) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

ACK2

Kat-Nr. : CL488-65094

Synonyme

Bs, c KIT, CD117, CD117 (c-Kit), Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, kit oncogene, SCO1, SCO5, SOW3, Ssm, Tr kit

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Galerie der Validierungsdaten

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  FC experiment of mouse bone marrow cells using CL488-65094

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with APC-Anti-Mouse CD11b (APC-65055, Clone: M1/70) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Mouse CD117 (c-Kit) (CL488-65094, Clone: ACK2). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL488-65094

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with APC-Anti-Mouse CD11b (APC-65055, Clone: M1/70) and CoraLite® Plus 488-conjugated Rat IgG2b isotype control antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65094 bindet in FC CD117 (c-Kit) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Murine IL-3-abhängige Mastzellen
• Vollständiger Name: kit oncogene
• GenBank-Zugangsnummer: BC052457
• Gene symbol: Kit
• Gene ID (NCBI): 16590
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).