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CD109 Polyklonaler Antikörper

CD109 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18715-1-AP

Synonyme

CD109, CD109 antigen, CD109 molecule, CPAMD7, p180, Platelet specific Gov antigen, r150, RP11 525G3.1

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of PC-3 cells using CD109 Polyclonal antibody (18715-1-AP)

    WB analysis of PC-3 using 18715-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18715-1-AP (CD109 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using CD109 Polyclonal antibody (18715-1-AP)

    IP experiment of PC-3 using 18715-1-AP

    IP Result of anti-CD109 (IP:18715-1-AP, 4ug; Detection:18715-1-AP 1:600) with PC-3 cells lysate 5000ug.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-3-Zellen
Erfolgreiche IPPC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

18715-1-AP bindet in WB, IP,ELISA CD109 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name CD109 molecule
Berechnetes Molekulargewicht 162 kDa
GenBank-ZugangsnummerBC152996
Gene symbol CD109
Gene ID (NCBI) 135228
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD109, also named as CPAMD7, is a member of the alpha2-macroglobulin/complement superfamily. It is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein of approximately 170kd which is found on the cell surface of platelets, activated T-cells, and endothelial cells. CD109 binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta). CD109 has been identified as part of the TGF-beta receptor system in human keratinocytes. Up regulation of CD109 expression has been observed in several different types of tumor.CD109 is a glycosyl-phosphatidylinositol-anchored glycoprotein that is a member of the a2-macroglobulin/C3, C4, C5 family of thioester-containing proteins. There're 2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. The antibody can recognize all these 4 isoforms.

Publikationen

SpeciesApplicationTitle
humanWB

Oncol Lett

CD109 expression is upregulated in penile squamous cell carcinoma.

Authors - Fengyun Dong
View Article