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CD107b / LAMP2 Monoklonaler Antikörper

CD107b / LAMP2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

ABL-93

Kat-Nr. : CL594-65052

Synonyme

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite®594 Anti-Mouse CD107b / LAMP2 (ABL-93) (CL594-65052)

    IF Staining of NIH/3T3 using CL594-65052

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite®594 CD107b antibody (CL594-65052, Clone: ABL-93 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite®594 Anti-Mouse CD107b / LAMP2 (ABL-93) (CL594-65052)

    FC experiment of NIH/3T3 using CL594-65052

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Mouse CD107b / LAMP2 (CL594-65052, Clone: ABL-93) (red), or 0.5 ug CoraLite®594 Rat IgG2a Isotype Control (2A3) (CL594-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFNIH/3T3-Zellen
Erfolgreiche Detektion in FCNIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-65052 bindet in IF, FC (Intra) CD107b / LAMP2 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Ratte / IgG2a, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Gereinigte Glykoproteine einer BALB/c-Mausembryo-3T3-Zelllinie
Vollständiger Name lysosomal-associated membrane protein 2
GenBank-ZugangsnummerBC138718
Gene symbol Lamp2
Gene ID (NCBI) 16784
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 CD107b / LAMP2 antibody CL594-65052Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen