CD107b Monoklonaler Antikörper

CD107b Monoklonal Antikörper für IF

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

IF

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

ABL-93

Kat-Nr. : CL555-65052

Synonyme

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

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Galerie der Validierungsdaten

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  IF Staining of NIH/3T3 using CL555-65052

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 555 CD107b antibody (CL555-65052, Clone: ABL-93 ) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-65052 bindet in IF CD107b und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigte Glykoproteine einer BALB/c-Mausembryo-3T3-Zelllinie
• Vollständiger Name: lysosomal-associated membrane protein 2
• GenBank-Zugangsnummer: BC138718
• Gene symbol: Lamp2
• Gene ID (NCBI): 16784
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen.

Hintergrundinformationen

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).