CD107b / LAMP2 Monoklonaler Antikörper

CD107b / LAMP2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

IF, FC (Intra)

Konjugation

APC Fluorescent Dye

CloneNo.

ABL-93

Kat-Nr. : APC-65052

Synonyme

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

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Galerie der Validierungsdaten

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  IF Staining of NIH/3T3 using APC-65052

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using APC-65052 (CD107b antibody) at dilution of 1:100.

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  FC experiment of NIH/3T3 using APC-65052

  1X10^6 NIH/3T3 cells were intracellularly stained with 10 ul APC Anti-Mouse CD107b (APC-65052, Clone:ABL-93) (red), or 10 ul Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

APC-65052 bindet in IF, FC (Intra) CD107b / LAMP2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigte Glykoproteine einer BALB/c-Mausembryo-3T3-Zelllinie
• Vollständiger Name: lysosomal-associated membrane protein 2
• GenBank-Zugangsnummer: BC138718
• Gene symbol: Lamp2
• Gene ID (NCBI): 16784
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und einem Stabilisator.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C